Biofunctionalization of Nanocomposites Ba¬sed on Magnetite Modified with Mezo-2,3-Dymer-captosuccin Acid

In this paper, we synthesize magnetite-based nanocomposites modified by meso-2,3-dymercaptosuccin acid with biologically active immobilized components (normal human immunoglobulin (Ig) and 2 mercaptonycotin acid (2-MNC). Also, we investigate physical-and chemical properties of magnetic suspensions based on them. We develop the method of chemical surface modification of single-domain magnetite with thiol groups. The obtained nanocomposite is biofunctionalized by normal human immoglobulin by using sulfo-SMCC heterofunctionalized reagent. We immobilize model biologically active compound 2-MNCs on the surface of magnetically sensitive nanocomposite by the reaction of thiols disulfide exchange using dipiridyl-disulfide. By employing XRT methods and Fourier infrared spectroscopy, we prove the presence of disulfide bridges and SH groups on the modified surface, and determine the quantitative content of thiol groups.

Publication year: 
2012
Issue: 
1
УДК: 
544.72.02
С. 149—154. Іл. 6. Табл. 1. Бібліогр.: 16 назв.
References: 

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