Isolation of Higher Basidiomycetes Prospective Producers of Biologically Active Substances in Pure Culture and Their Long-Term Storage

In this paper, we select methods and conditions for isolation of pure culture and long-term storage of three basidiomycetes species Schizophyllum commune Fr., Laetiporus sulphureus (Bull.) Murrill and Polyporus squamosus Huds.: Fr., which are promising producers of biologically active substances nowadays. We establish that the most effective methods of transferring strains in pure culture for S. commune was selection from spore material, and for L. sulphureus and P. squamosus – from basidiomes. 13 strains S. commune, 13 strains of L. sulphureus and 7 strains of P. squamosus are isolated from environmental conditions by using selected effective methods. We propose conditions for long-term safe strains keeping. Agar wort and temperature 4 °C are the best conditions for cultures of investigated species for 12 months and more. For 6 months of storage it is possible to use potato-glucose agar medium. The proposed methods allow keeping strains under industrials conditions without the loss of biological and technical specificity.

Publication year: 
2013
Issue: 
3
УДК: 
УДК 582.284.3
С. 12–17. Іл. 4. Табл. 1. Бібліогр.: 22 назви.
References: 

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References [transliteration]: 

1. Ch. Hobbs, Medicinal Mushrooms: An exploration of Tradition, Healing and Culture. Santa Cruz: Botanica Press, 1995, 251 p.
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