Creation of Lettuce Transgenic Plants Carrying Gene of the Mycobacterium Tuberculosis Antigens ESAT6:Ag85B Fusion Protein

Transgenic plants are promising and potentially safe tools to produce edible vaccines. Passive immunization by oral delivery of Mycobacterium tuberculosis antigens expressed in transgenic plants seems to be the perspective strategy to combat tuberculosis. In this paper, we discuss the generation of lettuce (Lactuca sativa) transgenic plants carrying the fused gene of Mycobacterium tuberculosis antigens ESAT6:Ag85B. By employing Agrobacterium mediated transformation method, transgenic plants are obtained. Vectors containing gene esxA fused with fbpB gene are used. Plasmid vectors also contain selective genes of neomycin phosphotransferase II (npt II) or phosphinothricin acetyl transferase (bar). Stable transgenic plants are selected. By using PCR analysis, we confirm the presence of target and selective genes in plants genome. Rooted plats are transferred to soil in the greenhouse for further experiments.

Publication year: 
2013
Issue: 
3
УДК: 
577.222.7:581.1
С. 65–69. Іл. 4. Табл. 1. Бібліогр.: 18 назв.
References: 

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