Allelic Polymorphism Research of Barley Genes Bmy1 and LOX-1 that are Related to Brewing Characteristics of Grain

This work was aimed at allelic status identification of the barley genes Bmy1 and LOX-1 in the collection, consisted of 103 domestic and foreign cultivars, and also selection lines. Research of the collection was held using polymerase chain reaction (PCR) with a subsequent analysis of gene LOX-1 allelic polymorphism via restriction of amplification products. Results of the PCR and restriction were visualized using gel-electrophoresis method. It was defined that 63 barley cultivars would be promising for brewing because of their middle activity and thermostability of β-amylase in malt. There was not a single cultivar or selection line with allele loxA, which was responsible for non-active type of lipoxygenase enzyme in beer, in the barley collection. Obtained results indicate low frequency of brewing alleles’ occurrence. Further searching with the help of the newest molecular markers for allelic diversity identification of genes Bmy1 and LOX-1, and, additionally, expansion of the general collection of barley cultivars and selection lines, will allow better differentiation of cultivars and choose among them the most advantageous ones that will become raw material for quality beer.

Publication year: 
2014
Issue: 
3
УДК: 
575.113.2:633.16:663.421
С. 88–94., Іл. 2. Табл. 2. Бібліогр.: 10 назв.
References: 

1. S.E. Clark et al., “Effects of single nucleotide polymorphisms in beta-amylase1 alleles from barley on functional properties of the enzymes”, Plant Physiol. Biochem., vol. 41, no. 9, pp. 798—804, 2003.
2. S.H. Duke and C. A. Henson, “A comparison of barley malt amylolytic enzyme activities as indicators of malt sugar concentrations”, J. Am. Soc. Brew. Chem., vol. 67, pp. 99—111, 2009.
3. M.A. Vinje et al., “Utilization of different Bmy1 intron III alleles for predicting в-amylase activity and thermostability in wild and cultivated barley”, Plant Molecular Biological Report, vol. 28, is. 3, pp. 491—501, 2010.
4. J. Gunkel et al., “Effect of the malting barley variety (Hordeum vulgare L.) on fermentability”, J. Inst. Brew., vol. 108, pp. 355—361, 2002.
5. J.M. Erkkila, “Intron III-specific for screening of вamylase alleles in barley cultivars”, Plant Molecular Biology Reporter, vol. 17, pp. 139—147, 1999.
6. W. Zhang et al., “в-Amylase variation in wild barley accessions”, Breeding Sci., vol. 54, pp. 41—49, 2004.
7. G. Yang et al., “Purification and characterization of lipoxygenase isozymes in germinating barley”, Am. Assoc. Cereal Chem., vol. 70, pp. 589—595, 1993.
8. N. Hirota et al., “Characterization of lipoxygenase-1 null mutants in barley”, Theoretical & Applied Genetics, vol. 111, is. 8, pp. 1580—1584, 2005.
9. M.G. Murray and W.F. Thompson, “Rapid isolation of high molecular weight plant DNA”, Nucleic Acids Res., vol. 8, is. 19, pp. 4321—4325, 1980.
10. Державний реєстр сортів рослин придатних для поширення в Україні в 2013 році. — К.: Український інститут експертизи сортів рослин, 2013. — С. 30—37.

References [transliteration]: 

1. S.E. Clark et al., “Effects of single nucleotide polymorphisms in beta-amylase1 alleles from barley on functional properties of the enzymes”, Plant Physiol. Biochem., vol. 41, no. 9, pp. 798–804,
2003.
2. S.H. Duke and C. A. Henson, “A comparison of barley malt amylolytic enzyme activities as indicators of malt sugar concentrations”, J. Am. Soc. Brew. Chem., vol. 67, pp. 99–111, 2009.
3. M.A. Vinje et al., “Utilization of different Bmy1 intron III alleles for predicting β-amylase activity and thermostability in wild and cultivated barley”, Plant Molecular Biological Report, vol. 28, is. 3, pp. 491–501, 2010.
4. J. Gunkel et al., “Effect of the malting barley variety (Hordeum vulgare L.) on fermentability”, J. Inst. Brew., vol. 108, pp. 355–361, 2002.
5. J.M. Erkkilä, “Intron III-specific for screening of β-amylase alleles in barley cultivars”, Plant Molecular Biology Reporter, vol. 17, pp. 139–147, 1999.
6. W. Zhang et al., “β-Amylase variation in wild barley accessions”, Breeding Sci., vol. 54, pp. 41–49, 2004.
7. G. Yang et al., “Purification and characterization of lipoxygenase isozymes in germinating barley”, Am. Assoc. Cereal Chem., vol. 70, pp. 589–595, 1993.
8. N. Hirota et al., “Characterization of lipoxygenase-1 null mutants in barley”, Theoretical & Applied Genetics, vol. 111, is. 8, pp. 1580–1584, 2005.
9. M.G. Murray and W.F. Thompson, “Rapid isolation of high molecular weight plant DNA”, Nucleic Acids Res., vol. 8, is. 19, pp. 4321–4325, 1980.
10. Derz͡havnyĭ rei͡estr sortiv roslyn prydatnykh dli͡a poshyrenni͡a v Ukraïni v 2013 rot͡si. – K.: Ukraïns′kyĭ instytut ekspertyzy sortiv roslyn, 2013. – S. 30–37.

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