Buchalo А.S.

Cultivation of Higher Basidiomycetes Schizophyllum Commune on Agar Nutrient Mediums

The growth and morphological features of 8 strains of medicinal mushroom Schizophyllum commune Fr. from the Culture collection of Mushrooms of the N.G. Kholodny Institute of Botany National Аcademy of Sciences of Ukraine, including 3 strains dedicated by the authors in different regions of Ukraine, were studied. The researches were investigated on 14 agar media: wort agar, potato glucose agar, synthetic medium with L asparagine or L asparagine with thiamin, medium of Norkrans, Czapek medium, modified Murashige and Skoog medium without hormones, glucose pepton yeast medium.

Isolation of Higher Basidiomycetes Prospective Producers of Biologically Active Substances in Pure Culture and Their Long-Term Storage

In this paper, we select methods and conditions for isolation of pure culture and long-term storage of three basidiomycetes species Schizophyllum commune Fr., Laetiporus sulphureus (Bull.) Murrill and Polyporus squamosus Huds.: Fr., which are promising producers of biologically active substances nowadays. We establish that the most effective methods of transferring strains in pure culture for S. commune was selection from spore material, and for L. sulphureus and P. squamosus – from basidiomes. 13 strains S. commune, 13 strains of L. sulphureus and 7 strains of P.

Selection of Conditions of Submerged Cultivation of Grifola frondosa as the Basis for Development of Biotechnology for Obtaining Medicinal Prophylactic Preparations

The paper investigates the influence of sources of carbon and nitrogen, pH and components of nutrient mediums (beer wort, molasses, corn extract, peptone, extract of yeasts) on the accumulation of biomass and exopolysaccharides under cultivation of higher basidiomycetes mushroom of Grifola frondosa (Dicks: Fr.) S.F. Gray. Crucially, we propose complex nutrient mediums with glucose, ammonium nitrate, corn extract and molasses favorable for biosynthesis of exopolysaccharides and biomass.