Marynchenko L.V.

Creation of Lettuce Transgenic Plants Carrying Gene of the Mycobacterium Tuberculosis Antigens ESAT6:Ag85B Fusion Protein

Transgenic plants are promising and potentially safe tools to produce edible vaccines. Passive immunization by oral delivery of Mycobacterium tuberculosis antigens expressed in transgenic plants seems to be the perspective strategy to combat tuberculosis. In this paper, we discuss the generation of lettuce (Lactuca sativa) transgenic plants carrying the fused gene of Mycobacterium tuberculosis antigens ESAT6:Ag85B. By employing Agrobacterium mediated transformation method, transgenic plants are obtained. Vectors containing gene esxA fused with fbpB gene are used.

Stimulation of Accumulation of Biomass and Fermenting Activity of Saccharomyces cerevisiae Yeast Culture by Extra High Frequency Electromagnetic Irradiation

The optimum irradiation parameters of EHF EMR for Saccharomyces cerevisiae M-09 yeast culture are experimentally determined. In the alcoholic brew, fermented with the irradiated yeast, the accumulation of biomass, alcohol and the amount of carbon dioxide emissions increased compared to control. Simultaneously the amount of nonfermented sugars decreased. The yeast vigor for the filtered brew yeast, their glucosidase and zymase activity enhanced.