Morgun B.V.

Allelic Polymorphism Research of Barley Genes Bmy1 and LOX-1 that are Related to Brewing Characteristics of Grain

This work was aimed at allelic status identification of the barley genes Bmy1 and LOX-1 in the collection, consisted of 103 domestic and foreign cultivars, and also selection lines. Research of the collection was held using polymerase chain reaction (PCR) with a subsequent analysis of gene LOX-1 allelic polymorphism via restriction of amplification products. Results of the PCR and restriction were visualized using gel-electrophoresis method. It was defined that 63 barley cultivars would be promising for brewing because of their middle activity and thermostability of β-amylase in malt.

Detection of Allelic Variants of Wax Gene among National and Foreign Barley Varieties

The objective of the present study was to apply of the molecular marker system for detection of the allelic variants of the Wаx gene. Starch pasting properties are very important factors in malting, food processing and feed quality of barley cultivars. Therefore, it is important to control of the amylose/amylopectin ratio in the barley starch. The Wаx locus of barley (Hordeum vulgare L.) is responsible for the amylose endosperm biosynthesis.