Problems of Biotechnology

Component Selection of Nutrient Medium Based on Sugar Beet Pulp Extract for Cultivating Laetiporus Sulphureus

In this survey the possibility of using sugar beet pulp extract as the basis of growth medium for the cultivation basidiomycetes Laetiporus sulphureus (Bull.: Fr.) Murrill was evaluated. Positive results of evaluating the suitability of the medium based on this extract for cultivation on agar plates and in liquid media were obtained. Strain that is best adapted to the new source of nutrients was selected from three strains during a series of experiments. Component composition of nutrient medium to conduct further experiments with the selected strain was improved.

Allelic Polymorphism Research of Barley Genes Bmy1 and LOX-1 that are Related to Brewing Characteristics of Grain

This work was aimed at allelic status identification of the barley genes Bmy1 and LOX-1 in the collection, consisted of 103 domestic and foreign cultivars, and also selection lines. Research of the collection was held using polymerase chain reaction (PCR) with a subsequent analysis of gene LOX-1 allelic polymorphism via restriction of amplification products. Results of the PCR and restriction were visualized using gel-electrophoresis method. It was defined that 63 barley cultivars would be promising for brewing because of their middle activity and thermostability of β-amylase in malt.

Development of Synthetic Detergents with Antiseptic Effect

Influence of the main components of synthetic detergents on lytic activity of enzyme preparations of Cytorecifen and Cytorecifen-M was investigated in this work. On the basis of previously obtained results and the analysis of compositions of detergents the sodium carbonate, zeolite 4A, carboxymethylcellulose as well as superficially active substances synthanol and sulphanol were used. Lytic activity of the enzyme preparation and the powder synthetic washing means had been established by turbydimetric method by the ability of the lyses of Staphyloccus aureus 209 test-culture.

The Growth Rates and the Accumulation Dynamics of Riboflavin of Ray Fungus Eremothecium Ashbyi Guillier.

The aim of the scientific research was to study the cultural and biochemical characteristics of Eremothecium ashbyi, primarily due to the dynamics of accumulation of riboflavin. The growth rates and the accumulation dynamics of riboflavin in the culture liquid and mycelium of ray fungus Eremothecium аshbyi F340, which is perspective object for receipt of riboflavin by biotechnological method were investigated. Examining the culture E.

The Enzyme Lipase: Analysis of Industry Use, Producers, a Process for Preparing

The analysis and synthesis of information on the field of use, sources, mechanism of action and properties of the enzyme lipase was performed in a review. It is established that for sectors of the economy in which lipase successfully used or is planned to be used is constantly expanding. Performed patent search of lipase modern producers has found among these bacteria, actinomyces, yeast and microscopic fungi. The features of the formation process of culture media and cultivation of lipase producers profound way.

Immunomodulating Properties of Probiotics Based on Lactobacteria and Plant Components

Purpose of this work was determination of the immunomodulatory effects of base probiotic composition of lactic acid bacteria based on genus Lactobacillus and the complex probiotic with carbyuloza on an experimental model of intact mice. It was found, that injection of carbyuloza in the probiotic composition increased functional activity of phagocytic system – namely, absorbing activity of macrophages.

The Influence of Carbon and Nitrogen Sources in Nutrient Media on Biomass Accumulation by Basidiomycetes Medicinal Mushrooms Genus Trametes (Fr.)

The article investigates the impact of natural sources of carbon and nitrogen nutrition and their quantitative ratio of biomass accumulation by medicinal basidiomycetes of the genus Trametes. The objects of the study were 18 strains of basidiomycetes species T. versicolor, T. suaveolens, T. gibbosa, T. hirsuta, T. zonatus, T. pubescens, T. serialis, T. trogii from Institute of Botany of N.G. Kholodny Institute of botany National Academy of Sciences of Ukraine collection of mushrooms.

Reserching Electrical Activity of Hippocampal Neurons in Culture

It is shown in this paper that the presynaptic cell directly causes postsynaptic current in the postsynaptic neuron determines its amplitude. Combination of the electrophysiological techniques, mathematical tools, and сytochemical research allow deeper analysis and understanding of the differences in the mechanisms of generation series of action potentials and excitatory inhibitory neurons in the hippocampus.

Using of magnetic therapy for the treatment of cardiovascular diseases

Method for operational control and quantitative analysis of magnetic field treatment process dynamics was developed. The calculation of amplitude and phase index of form is offered for the signals of pulse wave, which allows quantitavely compare the degrees of difference of pulse waves, and has a sensitiveness to influence the magnetic field. Method for evaluating the effectiveness of the use of magnetic therapy based on registration and signal processing of pulse wave using the technology of neural networks and calculating the index form for each patient.

Agrobacteria as the Potential Producents of Magnetosensitive Nanostructures

The aim of the study was the identification of the homologues of the Mam proteins mediating the magnetite synthesis of Magnetospirillum gryphiswaldense MSR-1 in the proteome of agrobacteria and their host plants. The identification of the protein homologues was performed by pairwise alignment using the online-service BLAST.