Problems of Biotechnology

Mathematical Simulation of Methane Production in the Fermentation Process

In this paper a mathematical model for the study of the process for producing the methane fermentation when the content of acetic acid changes in the fermenter, which is formed during the methanogenesis, was proposed. At the core of the mathematical model calculation there is the ideal mixing reactor theory. As a raw material for the production of methane by microorganisms a mixture of poultry manure and waste corn was chosen. The fermentation was performed under anaerobic conditions with the temperature 37±2 °С.

Heat Shock Proteins: Role in Formation of Immune Response

Aim of this work was to analyze the results of scientific studies of biological properties of heat shock proteins of pro- and eukaryotic beings and to identify the mechanisms of their interaction with human immune system. Heat shock proteins have been founded in pro- and eukaryotes, they are conserved molecules produced by cells in response to stress, and are present in intrasellar space as well as in extracellular environment and under normal conditions. The most important biological function of these proteins is chaperon activity.

Cultivation of Higher Basidiomycetes Schizophyllum Commune on Agar Nutrient Mediums

The growth and morphological features of 8 strains of medicinal mushroom Schizophyllum commune Fr. from the Culture collection of Mushrooms of the N.G. Kholodny Institute of Botany National Аcademy of Sciences of Ukraine, including 3 strains dedicated by the authors in different regions of Ukraine, were studied. The researches were investigated on 14 agar media: wort agar, potato glucose agar, synthetic medium with L asparagine or L asparagine with thiamin, medium of Norkrans, Czapek medium, modified Murashige and Skoog medium without hormones, glucose pepton yeast medium.

Antagonistic Activity of Pseudomonas fluorescence 2303 Strains to Fitopathohenic Microorganisms

In this paper, we study the antagonism of Pseudomonas fluorescens 2303 strain on pathogenic bacteria’s and fungi’s. This strain inhibited the development of P.syringae pv. syringae 8511, P.syringae pv. atrofaciens 9400, Xantomonas campestris 8003b, Clavibacter michiganensis 102 – stunting zones were 20–40 mm. Strain P.fluorescens 2303, unlike the product haupsyn, inhibited the growth of pathogenic bacteria such as Pectobacterium carotovorum 8982 and 8626 Agrobacterium tumefaciens.

Volumetric Estimation of Biogas Content

In this paper, we develop the method of volumetric analysis to estimate biogas content due to using the gas analyzer (Gas Analyser Chemical Laboratorial-1). We propose the list of absorbing chemical solutions and sequence of their application, notably solution of 50 60 % sulfuric acid – for ammonia absorption; saturated solution of I2 in 0,1 М water solution of KI – for hydrogen sulphide absorption; and solution of 35 % potassium hydroxide – for carbon dioxide absorption.

Hypothesis about the Use of Ergot Sclerotia in the Old Russian Iconography

This article shows the importance of research on the nature of the materials used in the ancient iconography that gave a special glow of pure colors and the luminosity of the faces to the icon created in the XIV–XV centuries. The approach to use such materials for the icons was lost by the old masters in the second half of the XVI century and nowadays it is considered to be the greatest riddle of the Orthodox icons. There are almost no scientific publications discussing this issue.

Mathematical and Programming Models of Coexistence of Two “Predator–Prey” Type Populations

It is a theoretical investigation in the field of modeling of \ numbering populations. We analyze different models of populations interactions and find essential imperfections of these models. We propose the mathematical model of interaction of the simplest population structure interaction. This model takes into consideration the phenomenon of the predator satiety under conditions of sufficient prey. This phenomenon stipulates that the predator does not eat more than it needs for satiation. The classical model ignores the predator’s satiation.

Using Species Molecular Features of Vascular Plants in Modern Pharmaceutical Biotechnology

The paper offers promising investment destination for addressing VI-th biotech level – network biopharmaceutical local associations, “the principle of cluster triangle” based on intellectual investments in conservation three-dimensional biological activity structures and use of information capacity in the pharmaceutical nanoscale lignin components of vascular plants.

Газлифтный барботажный аппарат

The purpose of research is to analyze the operation of gas-lift bubble-type vehicle and clarify the nature of energy concentration areas of working liquid fluctuation motion under the impact of external acoustic radiation. The effect of cultural liquid indignation inside the vehicle’s working volume has an indisputable advantage. This effect can be achieved without mechanical mixing devices and technological deficiencies arising out of their use.

Creation of Lettuce Transgenic Plants Carrying Gene of the Mycobacterium Tuberculosis Antigens ESAT6:Ag85B Fusion Protein

Transgenic plants are promising and potentially safe tools to produce edible vaccines. Passive immunization by oral delivery of Mycobacterium tuberculosis antigens expressed in transgenic plants seems to be the perspective strategy to combat tuberculosis. In this paper, we discuss the generation of lettuce (Lactuca sativa) transgenic plants carrying the fused gene of Mycobacterium tuberculosis antigens ESAT6:Ag85B. By employing Agrobacterium mediated transformation method, transgenic plants are obtained. Vectors containing gene esxA fused with fbpB gene are used.