Problems of Biotechnology

Impact of Biogenic Metals Citrates Obtained by Aquananotechnology, on Growth and Biosynthetic Activity of Medicinal Mushroom Tra¬metes Versicolor

We conduct the comparative study of the impact of biogenic metals citrates such as zinc, magnesium, iron obtained using nanotechnology, on growth of mycelium and biosynthetical activity of medicinal fungi Trametes versicolor at their cultivation in a liquid medium. To characterize the biosynthetic activity, we determine mass fraction of organic acids, exopolysaccharides concentration of phenol-sulfate method, the protein content by Lowry, cellulolytic activity in carboxymethylcellulose (CMC-activity) and filter paper (FP-activity).

Production of Enzymic Preparations in Ukraine

In this paper, we analyze the modern state of production enzymic preparations in Ukraine. Based on the source, enzymic preparations are classified as industrial (produced from microbal raw material) and medical (produced mainly from animal raw material). According to the data of Abercade Consulting, we present the growth dynamics of global market of enzymic preparations. Specifically, we show that the enzymic preparations production in Ukraine greatly depends on import. We determine leading enzyme producing companies, which import prodcuts to Ukraine and CIS countries.

Application of Magnetically Labeled Cells S. Cerevisiae as Biosorbents at Treatment Plants

This paper studies the sorption capacity of magnetically labeled biosorbents – yeast cells Saccharomyces cerevisiae – to remove iron ions (II). The study is conducted on model solutions and wastewater samples from Slavutych, Chernihiv region. We obtain magnetically treated biosorbents by modifying Saccharomyces cerevisiae baker yeast by magnetite nanoparticles. We perform modification by mechanical and multivertex magnetohydrodynamic mixing (MHDM) of yeasts’ suspensions with magnetite nanoparticles.

Ferritine and biomineralization of biogenic magnetic nanoparticles in microorganisms

In this paper, we verify the hypothesis on obligatory participation of ferritin molecules in the biomineralization process of biogenic magnetic nanoparticles assuming that the genetic mechanism of biogenic magnetic nanoparticles is consistent for prokaryotes and eukaryotes. Using comparative genomic methods, we seek to reveal whether all the magnetic bacteria have ferritin genes in their genome. Well known proteins (ferritin and ferritin-like proteins) are compared with compiling magnetotactice bactria genomes using the blastn “BLAST on-line” under standard program parameters.

Biomineralization of Intracellular Biogenic Magnetic Nanoparticles and Their Expected Functions

The purpose of this paper is to analyze the similarity between proteins of MI MTB and organisms genomes of three main kingdoms: bacteria, archea and eukaryotes for revealing the joint genetic basis of biomineralization of intracellular biogenic magnetic nanoparticles (IBMN). In addition, the research objective is to estimate the energy of paramagnetic and efficiently paramagnetic energy of cluster components compared with their thermal motion energy for establishing the physical mechanism of possible influence of nonuniform in a magnetic field of IBMN on cell metabolism.

Resistance Determination of a Probiotic Strain Bacteria to Antibiotics

The objective of research is to determine sensitivity of a probiotic strain to a number of antibiotics which are frequently used in the veterinary practice. To that end, we employ disk-diffusion method in the MRS-agar and used standard antibiotics-soaked paper discs. The results interpretation conform to literary sources. Specifically, Lactobacilus rhamnosus; L.acidophilus; L.brevis; L.paracasei ssp. paracasei; L.casei; L.delbrueckii subsp.

Isolation of Higher Basidiomycetes Prospective Producers of Biologically Active Substances in Pure Culture and Their Long-Term Storage

In this paper, we select methods and conditions for isolation of pure culture and long-term storage of three basidiomycetes species Schizophyllum commune Fr., Laetiporus sulphureus (Bull.) Murrill and Polyporus squamosus Huds.: Fr., which are promising producers of biologically active substances nowadays. We establish that the most effective methods of transferring strains in pure culture for S. commune was selection from spore material, and for L. sulphureus and P. squamosus – from basidiomes. 13 strains S. commune, 13 strains of L. sulphureus and 7 strains of P.

Development of improved method of Isolation and Purification of human IgE

We aim at creating and evaluating the acceptability of improved methods of isolation of high purity human IgE suitable for use in highly sensitive immuno-analytical methods. To this end, we generalize physicochemical and biological properties of immunoglobulins of different classes and analyze key methodological techniques used for isolation and purification of human IgE (ion exchange chromatography on DEAE-cellulose, gel filtration on Sephadex G-200 and S-300 Sephacryl different options immunoaffinity chromatography).

The Growth Kinetics of Lactic Acid Bacteria Genum Lactobacillus on Nutrient Media with Different Sources of Carbon and Nitrogen Nutrition

The study assesses the possibility of using biotechnology probiotic soy and glucose-pyruvate culture media, which contain different sources of carbon and nitrogen. When choosing nutrient media, we take into account the end product cost, which can be reduced by decreasing the cost of medic components and simplifying its preparation. We investigate five strains of lactic acid bacteria p. Lactobacillus serving the basis of a new probiotic.

Alternative Components of Nutrient Mediums for Actinomycetes – Producer of Biologically Active Substances

Taking into account the wide-spread of such group as actinomycetes among industrial producers, we use one of its representatives (Streptomyces recifensis var. lytіcus – a producer of a lytic enzyme complex) as a model for studying alternative components which can be utilized for optimization of nutrient mediums for actinomycetes. We study the influence of alternative components of nutrient mediums on biosynthetic processes of culture Str. recifensis var. lytіcus and define the possibility of their use for optimization of nutrient mediums for actinomycetes.