Problems of Biotechnology

Influence of Glucose Concentration in Culture Medium on Recombinant gp 120 HIV-1 Production

Contingent screening for specific serological markers of infection ranks high in HIV prophylaxis. Nowadays the most common serological screening practice of HIV is contingent testing to detect serum antibodies against the HIV using an enzyme linked immunosorbent assay (ELISA). Recombinant analogues of HIV-1 envelope protein gp 120 are widely used in all commercial ELISA test kits for HIV diagnostics. In this paper we study the production of rgp 120 in E.coli host strain. The research strategies focus on improving the cultivation techniques and manipulating the bacteria physiology.

Ways of Forming Biofilm of Microorganisms with Exoelectrogens Activity

The development and enhancement of bioelectrochemical systems to produce electricity and hydrogen is a promising area of modern bioenergy. Microorganisms are the active component of bioelectrochemical process in bioelectrochemical systems. These microorganisms further the processes of degradation of energy substrate to form compounds and exocell transfer of electrons to a terminal acceptor. We analyze existing methods of biofilm formation of electroactive microorganisms depending on its species diversity and processes of formation.

Development of Improved Method of Obtaining of Fc-fragments of Human IgM

The aim of work was to develop the improved methods of obtaining and allocating Fc-fragments of human IgM. To achieve this goal IgH hydrolysis with subsequent purification (Fс)5μ-fragments was optimized.

Stimulation of Accumulation of Biomass and Fermenting Activity of Saccharomyces cerevisiae Yeast Culture by Extra High Frequency Electromagnetic Irradiation

The optimum irradiation parameters of EHF EMR for Saccharomyces cerevisiae M-09 yeast culture are experimentally determined. In the alcoholic brew, fermented with the irradiated yeast, the accumulation of biomass, alcohol and the amount of carbon dioxide emissions increased compared to control. Simultaneously the amount of nonfermented sugars decreased. The yeast vigor for the filtered brew yeast, their glucosidase and zymase activity enhanced.

Biohydrogen Production Ways

The present paper describes biohydrogen production methods according to the classification of energy sources used by microorganisms. We also conduct the comparative analysis of these processes. We describe the microorganism-producers, hydrogen formation reaction and optimal environmental parameters for every method of biohydrogen production. All these methods have their advantages and disadvantages. Crucially, we define the most optimal biohydrogen production way. Photobiological hydrogen production and biomass production should take place on the first stage.

Self-Organization of Magnetic Nanoparticles When Giving Magnetic Properties to Yeast Saccharomyces Cerevisiae

We develop and conduct the compararative analysis of four evaluation methods of clusters parameters of magnetic nanoparticles. The magnetically-controllable biosorbent based on the S. cerevisiae culture is used as a model object for analyzing the adequacy of these models. Finally, we obtain the evaluation data on the depth of clusters penetration inside the biomembrane, typical sizes of clusters and dispersion of magnetic cluster sizes. Our analysis shows that all four methods can be used for single magnetic clusters.

Increasing the Microbial Pathogen Stability as the Factor of the Development of Novel Antiseptic Means

This paper demonstrates the role of major mechanisms of resistance and additional factors in the process of increasing the stability of microbial pathogenes. We describe the approaches to prevention of microorganisms resistance formation as well as ways and perspectives of developing novel antiseptic means. Among promising directions of new antiseptics development are preparations that specifically inhibit the mechanisms of cells growth (QS-system etc.), bacteriophage substances and its derivatives, modified bacteriolytic enzymes.

Selection of Conditions of Submerged Cultivation of Grifola frondosa as the Basis for Development of Biotechnology for Obtaining Medicinal Prophylactic Preparations

The paper investigates the influence of sources of carbon and nitrogen, pH and components of nutrient mediums (beer wort, molasses, corn extract, peptone, extract of yeasts) on the accumulation of biomass and exopolysaccharides under cultivation of higher basidiomycetes mushroom of Grifola frondosa (Dicks: Fr.) S.F. Gray. Crucially, we propose complex nutrient mediums with glucose, ammonium nitrate, corn extract and molasses favorable for biosynthesis of exopolysaccharides and biomass.

Modeling of the Technological Process for Live Rotavirus Vaccine Production

We simulate the industrial technology for rotavirus vaccine, based on the strain of group A rotavirus with G1P[8] genotype, circulating among people in Ukraine. We propose to use this strain of rotavirus as the industrial strain. Through successive passages, it is adapted to cultivation conditions in the continuous human and animal cell cultures. The model of technology for the rotavirus vaccine, based on the industrial strain of rotavirus, is developed for its cultivation in continuous cell culture Vero.

Medicinal Xylotrophic Basidiomycetes Laetiporus Sulphureus (Bull.: Fr.) Murill – Perspective Object of Biotechnology

This paper brings into light the critical review of publications on the chemical composition, action, and the use of compounds isolated from fruit bodies, submerged mycelium and culture liquid of edible basidiomycete Laetiporus sulphureus. In addition, we uncover biologically active compounds revealing the role of L. sulphureus as a substances producer that can be applied in various fields of industry.