Создание трансгенных растений салата, содержащих ген сшитого белка антигенов ESAT6:Ag85B из Mycobacterium Tuberculosis

Целью работы является анализ сходства всех белков магнитосомного островка магнитотаксисных бактерий с геномами организмов трех основных царств: бактерий, архей и эукариот, для выявления возможной общей генетической основы механизма биоминерализации внутриклеточных биогенных магнитных наночастиц (ВБМН). Также целью работы является оценка энергии как парамагнитных, так и эффективно парамагнитных внутриклеточных кластерных компонент в магнитном поле ВБМН в сравнении с энергией их теплового движения для установления физического механизма возможного влияния неоднородных магнитных полей ВБМН на метаболизм в клетке. При использовании методов биоинформатики показано, что существует общая генетическая основа биоминерализации ВБМН в разных организмах, геномы которых известны. Также показано, что энергия как парамагнитных, так и эффективно парамагнитных внутриклеточных кластерных компонент в магнитном поле ВБМН может значительно превышать энергию их теплового движения. Это позволяет осуществлять их направленный транспорт под воздействием неоднородных магнитных полей ВБМН внутри клетки. Поэтому ВБМН могут представлять собой внутриклеточную магнитную наномашину для управления транспортными процессами в клетке, в частности производством активных форм кислорода, что в свою очередь может влиять на функционирование иммунной системы, передачу клеточных сигналов, запуск различных сигнальных систем, биосинтез ряда белков, обонятельную и осязательную рецепцию, регуляцию давления и т.д.

Год издания: 
2013
Номер: 
3
УДК: 
57.05
С. 28–33. Бібліогр.: 41 назва.
Литература: 

1. R.P. Blakemore, “Magnetotactic bacteria”, Sci., vol.190, pp. 377—379, 1975.
2. D.A. Kuterbach and B. Walcott, “Iron-containing cells in the honey-bee (Apis mellifera). I. Adult morphology and physiology”, J. Exp. Biol., vol. 126, pp. 375—87, 1986, pp. 389—401.
3. A. Bharde et al., “Sastry Extracellular Biosynthesis of Magnetite using Fungi”, Small., vol. 2, pp. 135—141, 2006.
4. S. Mann et al., “Morphology and organization of biogenic magnetite from sockeye salmon, Oncorhynchus nerka: Implications for magnetoreception”, J. Exp. Biol., vol. 140, pp. 35—49, 1988.
5. C. Walcott et al., “Pigeons have magnets”, Sci., vol. 184, pp. 180—182, 1979.
6. J. Zoeger et al., “Magnetic material in the head of the common Pacific dolphin”, Ibid, vol. 213, pp. 892—894, 1981.
7. J.L. Kirschvink et al., Magnetite biomineralization and magnetoreception in organisms: a new biomagnetism, New York: Plenum Press, 1985, 682 p.
8. P.P. Schultheiss-Grassi and J. Dobson, “Magnetic analysis of human brain tissue”, BioMetals, vol. 12, pp. 67—72, 1999.
9. P.P. Schultheiss-Grassi et al., “Analysis of magnetic material in the human heart, spleen and liver”, Ibid, vol. 10, pp. 351—355, 1997.
10. F. Brem et al., “Magnetic iron compounds in the human brain: a comparison of tumor and hippocampal tissue”, J. R. Soc. Interface, vol. 3, pp. 833—841, 2006.
11. S. Ullrich et al., “Hypervariable 130-Kilobase Genomic Region of Magnetospirillum gryphiswaldense Comprises a Magnetosome Island Which Undergoes Frequent Rearrangements during Stationary Growth”, J. Bacteriol, vol. 187, pp. 7176—7184, 2005.
12. A. Fernanda et al., “Common ancestry of iron oxide- and iron-sulfide-based biomineralization in magnetotactic bacteria”, The ISME J., vol. 5, pp. 1634—1640, 2011.
13. S.V. Gorobets and O.Yu. Gorobets,“Functions of biogenic magnetic nanoparticles in organisms”, Functional Mater., vol. 19, pp. 18—26, 2012.
14. Yu.I. Gorobets and S.V. Gorobets,“Stationary flows of electrolytes in the vicinity of ferromagnetic particles in a constant magnetic field”, Bull. of Herson State Tech. Un., vol. 3, no. 9, pp. 276—281, 2000.
15. S. Ullrich et al., “Functional Analysis of the Magnetosome Island in Magnetospirillum gryphiswaldense: The mamAB Operon Is Sufficient for Magnetite Biomineralization”, PLoS One, vol. 6, no. 10, 2011.
16. S. Schübbe et al., “Transcriptional Organization and Regulation of Magnetosome Operons in Magnetospirillum gryphiswaldense”, Appl. Environ. Microbiol.,vol. 72, no. 9, pp. 5757—5765, 2006.
17. H. Nakazawa et al., “Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria”, Genome Res., vol. 19, pp. 1801—1808, 2009.
18. A.P. Taylor and J.C. Barry, “Magnetosomal matrix: ultrafine structure may template biomineralization of magnetosomes”, J. Microsc., vol. 213, pp. 180—197, 2004.
19. J.L. Kirschvink et al.,“Magnetite-based magnetoreception”, Sensory systems, vol. 11, pp. 462—468, 2001.
20. K.W. Mandernack et al.,“Oxygen and iron isotope studies of magnetite produced by magnetotactic bacteria”, Sci., vol. 285, pp. 1892—1896, 1999.
21. Biello D.,“The Origin of Oxygen in Earth’s Atmosphere: The breathable air we enjoy today originated from tiny organisms, although the details remain lost in geologic time”, Sci. American, August 2009.
22. L.M. Tiede et al., “Oxygen matters: tissue culture oxygen levels affect mitochondrial function and structure as well as responses to HIV viroproteins”, Cell. Death Dis., vol. 2, p. 246, 2011.
23. E. Ortiz-Prado et al., “A method for measuring brain partial pressure of oxygen in unanesthetized unrestrained subjects: the effect of acute and chronic hypoxia on brain tissue PO2”, J. Neurosci Methods, vol. 193, no. 2, p. 217,
24. S. Osinsky and P.Vaupel, “Tumor Microphysiology. Metabolic Microenvironment of Tumor Cells: Characteristics, Impact on Tumor Progression, Clinical Implications”, in Sci. Book Project, Ukraine, Kiev: Naukova Dumka, 2009.
25. F.F. Guo et al. (2012). Magnetosomes eliminate intracellular reactive oxygen species in Magnetospirillum gryphiswaldense MSR-1 [Online]. Available: http://onlinelibrary. wiley.com.sci-hub.org doi/10.1111/j.14622920.2012.02707.x/full
26. P. Ma et al. (2012). Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces hepatic and renal tissue injury via oxidative stress in mice [Online]. Available: http:// www. ncbi.nlm.nih.gov.scihub.org/pmc/-articles/ PMC3439859
27. P. Houdy et al., Nanoethics and nanotoxicology, Germany, Berlin: Springer Verlag, p. 620, 2011.
28. M. Winklhofer and N. Petersen, Paleomagnetism and Magnetic Bacteria, Germany, Berlin: Springer-Verlag, 2006, pp. 256—273.
29. O.Yu. Gorobets et al., “Quasi-stationary heterogeneous states of electrolyte at electrodeposition and etching process in a gradient magnetic field of a magnetized ferromagnetic ball”, J. MMM, vol. 330, pp. 76—80, 2013.
30. Yu.I. Gorobet and S.V. Gorobets, “Formation of stationary flows of liquid in visinity of ferromagnetic packing in constant magnetic field”, J. MHD, vol. 36, pp. 75—78, 2000.
31. K. Zhu et al., “Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea”, Res. Microbiol., vol. 161, pp. 276—283, 2010.
32. F.J. Friedlaender et al., “Particle Motion Near and Capture on Single Spheres in HGMS”, IEEE Trans. Magn., vol. 17, no. 6, pp. 2801—2803, 1981.
33. Павлович Н.В. Магнитная восприимчивость организмов. — М.: Наука и техника, 1985. — 111 c.
34. D.A. Bazylinski, “Synthesis of the bacterial magnetosome: the making of a magnetic personality”, Int. Microbiol., vol. 2, pp. 71—80, 1999.
35. D.A. Bazilynski et al., “Controlled Biomineralization of Magnetite (Fe3O4) and Greigite (Fe3S4) in a Magnetotactic Bacterium”, J. AEM, vol. 61, no. 9, pp. 3232— 3239, 1995.
36. M. Kajimura et al., “Interactions of Multiple Gas-Transducing Systems: Hallmarks and Uncertainties of CO, NO, and H2S Gas Biology”, J. ARS, vol. 13, no. 2, pp. 157—192, 2010.
37. I. Bertini et al., “NMR Spectroscopy of Paramagnetic Metalloproteins”, J. Chem. Bio. Chem., vol. 6, pp. 1536— 1549, 2005.
38. S. Klein et al., “Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation”, J. BBRC., vol. 425, no. 2, pp. 393—397, 2012.
39. Ганшин В.М., Лабас Ю.А., Зинкевич Э.П. Возможная роль активных форм кислорода в первичных механизмах обонятельной рецепции // Сенсорные системы. — 2010. — 24. — С. 74—93.
40. X. Wang and L .Liang, “Effects of Static Magnetic Field on Magnetosome Formation and Expression of mamA, mms13, mms6 and magA in Magnetospirillum magneticum AMB-1”, J. BEMS, vol. 30, pp. 313—321, 2009.
41. Y. Cui et al. (2012). Deficits in Water Maze Performance and Oxidative Stress in the Hippocampus and Striatum Induced by Extremely Low Frequency Magnetic Field Exposure [Online]. Available: http://www.plosone.org/article/- info%3Adoi%2F10.1371%2Fjournal.pone.0032196

Транслитерированый список литературы: 

1. R.P. Blakemore, “Magnetotactic bacteria”, Sci., vol.190, pp. 377–379, 1975.
2. D.A. Kuterbach and B. Walcott, “Iron-containing cells in the honey-bee (Apis mellifera). I. Adult morphology and physiology”, J. Exp. Biol., vol. 126, pp. 375–87, 1986, pp. 389–401.
3. A. Bharde et al., “Sastry Extracellular Biosynthesis of Magnetite using Fungi”, Small., vol. 2, pp. 135–141, 2006.
4. S. Mann et al., “Morphology and organization of biogenic magnetite from sockeye salmon, Oncorhynchus nerka: Implications for magnetoreception”, J. Exp. Biol., vol. 140, pp. 35–49, 1988.
5. C. Walcott et al., “Pigeons have magnets”, Sci., vol.184, pp. 180–182, 1979.
6. J. Zoeger et al., “Magnetic material in the head of the common Pacific dolphin”, Ibid, vol. 213, pp. 892–894, 1981.
7. J.L. Kirschvink et al., Magnetite biomineralization and magnetoreception in organisms: a new biomagnetism, New York: Plenum Press, 1985, 682 p.
8. P.P. Schultheiss-Grassi and J. Dobson, “Magnetic analysis of human brain tissue”, BioMetals, vol. 12, pp. 67–72, 1999.
9. P.P. Schultheiss-Grassi et al., “Analysis of magnetic material in the human heart, spleen and liver”, Ibid, vol. 10, pp. 351–355, 1997.
10. F. Brem et al., “Magnetic iron compounds in the human brain: a comparison of tumor and hippocampal tissue”, J. R. Soc. Interface, vol. 3, pp. 833–841, 2006.
11. S. Ullrich et al., “Hypervariable 130-Kilobase Genomic Region of Magnetospirillum gryphiswaldense Comprises a Magnetosome Island Which Undergoes Frequent Rearrangements during Stationary Growth”, J. Bacteriol, vol. 187, pp. 7176–7184, 2005.
12. A. Fernanda et al., “Common ancestry of iron oxide- and iron-sulfide-based biomineralization in magnetotactic bacteria”, The ISME J., vol. 5, pp. 1634–1640, 2011.
13. S.V. Gorobets and O.Yu. Gorobets,“Functions of biogenic magnetic nanoparticles in organisms”, Functional Mater., vol. 19, pp. 18–26, 2012.
14. Yu.I. Gorobets and S.V. Gorobets,“Stationary flows of electrolytes in the vicinity of ferromagnetic particles in a constant magnetic field”, Bull. of Herson State Tech. Un., vol. 3, no. 9, pp. 276–281, 2000.
15. S. Ullrich et al., “Functional Analysis of the Magnetosome Island in Magnetospirillum gryphiswaldense: The mamAB Operon Is Sufficient for Magnetite Biomineralization”, PLoS One, vol. 6, no. 10, 2011.
16. S. Schübbe et al., “Transcriptional Organization and Regulation of Magnetosome Operons in Magnetospirillum gryphiswaldense”, Appl. Environ. Microbiol.,vol. 72, no. 9, pp. 5757–5765, 2006.
17. H. Nakazawa et al., “Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria”, Genome Res., vol. 19, pp. 1801–1808, 2009.
18. A.P. Taylor and J.C. Barry, “Magnetosomal matrix: ultrafine structure may template biomineralization of magnetosomes”, J. Microsc., vol. 213, pp. 180–197, 2004.
19. J.L. Kirschvink et al.,“Magnetite-based magnetoreception”, Sensory systems, vol. 11, pp. 462–468, 2001.
20. K.W. Mandernack et al.,“Oxygen and iron isotope studies of magnetite produced by magnetotactic bacteria”, Sci., vol. 285, pp. 1892–1896, 1999.
21. Biello D.,“The Origin of Oxygen in Earth’s Atmosphere: The breathable air we enjoy today originated from tiny organisms, although the details remain lost in geologic time”, Sci. American, August 2009.
22. L.M. Tiede et al., “Oxygen matters: tissue culture oxygen levels affect mitochondrial function and structure as well as responses to HIV viroproteins”, Cell. Death Dis., vol. 2, p. 246, 2011.
23. E. Ortiz-Prado et al., “A method for measuring brain partial pressure of oxygen in unanesthetized unrestrained subjects: the effect of acute and chronic hypoxia on brain tissue PO2”, J. Neurosci Methods, vol. 193, no. 2, p. 217, 2010.
24. S. Osinsky and P.Vaupel, “Tumor Microphysiology. Metabolic Microenvironment of Tumor Cells: Characteristics, Impact on Tumor Progression, Clinical Implications”, in Sci. Book Project, Ukraine, Kiev: Naukova Dumka, 2009.
25. F.F. Guo et al. (2012). Magnetosomes eliminate intracellular reactive oxygen species in Magnetospirillum gryphiswaldense MSR-1 [Online]. Available: http:/ onlinelibrary.wiley.com.sci-hub.org/doi/10.1111 j.14622920.2012.02707.x/full
26. P. Ma et al. (2012). Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces hepatic and renal tissue injury via oxidative stress in mice [Online]. Available: http: www.ncbi.nlm.nih.gov.scihub.org/pmc/-articles/PMC3439859
27. P. Houdy et al., Nanoethics and nanotoxicology, Germany, Berlin: Springer Verlag, p. 620, 2011.
28. M. Winklhofer and N. Petersen, Paleomagnetism and Magnetic Bacteria, Germany, Berlin: Springer-Verlag, 2006, pp. 256–273.
29. O.Yu. Gorobets et al., “Quasi-stationary heterogeneous states of electrolyte at electrodeposition and etching process in a gradient magnetic field of a magnetized ferromagnetic ball”, J. MMM, vol. 330, pp. 76–80, 2013.
30. Yu.I. Gorobet and S.V. Gorobets, “Formation of stationary flows of liquid in visinity of ferromagnetic packing in constant magnetic field”, J. MHD, vol. 36, pp. 75–78, 2000.
31. K. Zhu et al., “Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea”, Res. Microbiol., vol. 161, pp. 276–283, 2010.
32. F.J. Friedlaender et al., “Particle Motion Near and Capture on Single Spheres in HGMS”, IEEE Trans. Magn., vol. 17, no. 6, pp. 2801–2803, 1981.
33. Pavlovich N.V. Magnitnai͡a vospriimchivost' organizmov. – M.: Nauka i tekhnika, 1985. – 111 s.
34. D.A. Bazylinski, “Synthesis of the bacterial magnetosome: the making of a magnetic personality”, Int. Microbiol., vol. 2, pp. 71–80, 1999.
35. D.A. Bazilynski et al., “Controlled Biomineralization of Magnetite (Fe3O4) and Greigite (Fe3S4) in a Magnetotactic Bacterium”, J. AEM, vol. 61, no. 9, pp. 3232–3239, 1995.
36. M. Kajimura et al., “Interactions of Multiple Gas-Transducing Systems: Hallmarks and Uncertainties of CO, NO, and H2S Gas Biology”, J. ARS, vol. 13, no. 2, pp. 157–192, 2010.
37. I. Bertini et al., “NMR Spectroscopy of Paramagnetic Metalloproteins”, J. Chem. Bio. Chem., vol. 6, pp. 1536–1549, 2005.
38. S. Klein et al., “Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation”, J. BBRC., vol. 425, no. 2, pp. 393–397, 2012.
39. Ganshin V.M., Labas I͡U.A., Zinkevich Ė.P. Vozmozhnai͡a rol' aktivnykh form kisloroda v pervichnykh mekhanizmakh oboni͡atel'noĭ ret͡sept͡sii // Sensornye sistemy. – 2010. – 24. – S. 74–93.
40. X. Wang and L .Liang, “Effects of Static Magnetic Field on Magnetosome Formation and Expression of mamA, mms13, mms6 and magA in Magnetospirillum magneticum AMB-1”, J. BEMS, vol. 30, pp. 313 321, 2009.
41. Y.Cui et al. (2012). Deficits in Water Maze Performance and Oxidative Stress in the Hippocampus and Striatum Induced by Extremely Low Frequency Magnetic Field Exposure [Online]. Available: http://www.plosone.org/article/-info%3Ado %2F10.1371%2Fjournal.pone.0032196

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